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INDONESIAN JOURNAL OF PHARMACY

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Published by Universitas Gadjah Mada

ISSN : 23389427 EISSN : 23389486 DOI : -

Core Subject : Health,

Medicine & Pharmacology

Indonesian Journal of Pharmacy (ISSN-e: 2338-9486, ISSN-p: 2338-9427), formerly Majalah Farmasi Indonesia (ISSN: 0126-1037). The journal had been established in 1972, and online publication was begun in 2008. Since 2012, the journal has been published in English by Faculty of Pharmacy Universitas Gadjah Mada (UGM) Yogyakarta Indonesia in collaboration with IAI (Ikatan Apoteker Indonesia or Indonesian Pharmacist Association) and only receives manuscripts in English. Indonesian Journal of Pharmacy is Accredited by Directorate General of Higher Education (DGHE) DIKTI No. 58/DIKTI/Kep/2013.

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Articles 9 Documents

Search results for , issue "Vol 24 No 2, 2013" : 9 Documents clear

TASTE MASKING OF DONEPEZIL HYDROCHLORIDE USING DIFFERENT ION EXCHANGE RESINS- A COMPARATIVE STUDY Zainuddin, Rana; Kulkarni, Alpana; Chavan, Hemant; Patil, Gunjan; Zaheer, Zahid; Sangshetti, Jaiprakash N.
INDONESIAN JOURNAL OF PHARMACY Vol 24 No 2, 2013
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Taste mainly depends on the physiology, sensitivity and structure of taste buds. It is an important parameter in administering drugs orally. Bitter taste is a major limitation to patient compliance. Donepezil hydrochloride (DH) is a bitter drug used in Alzheimers disease. Amongst the many techniques for taste masking, using ion exchange resins has been extensively reported. The technique of forming tasteless complexes with bitter drugs involves selection of most appropriate exchanger and optimization of complexing ratio. The aim of the present work was to select the best cationic exchanger amongst Indion 414, Indion 234 and Indion 214. All parameters were optimized to produce drug-loaded tasteless complexes. Complexation was carried out using batch process prior to which, acid-alkali activation was performed to remove adsorbed impurities from the resin bed surface and hence improve loading efficiency. UV-spectrophotometric method was used to determine percent drug loading. The molecular properties of drug resin complexes were studied using Fourier Transform Infra-red Spectroscopy, Differential Scanning Calorimetry and Xray Powder Diffraction which confirmed complexation. Indion 414 was found to give highest drug loading and minimal drug was released from the complex at salivary pH. Key words: Donepezil HCl, Ion exchange resins, Molecular properties, Taste masking

SELECTION OF pEGFP-c1-TRANSFECTED-CHO-K1 CELLS BY G418 DECREASED THE EXPRESSION OF GREEN FLUORESCENT PROTEIN Endah Puji Septisetyani; Adi Santoso
Indonesian Journal of Pharmacy Vol 24 No 2, 2013
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

The most common proteins used for reporter protein is the green fluorescent protein (GFP). It is very convenient to detect the GFP fluorescent by fluorescent microscopy or flowcytometry to monitor the successful transfection. The gfp gene can be introduced into the cells by transfecting of two different plasmid vectors or one vector containing both gfp and the gene of our interest. In this current experiment, we used pEGFP-c1 plasmid to express gfp in CHO-K1 cells. We transfected the CHO-K1 cells by using cationic lipid Lipofectamin 2000. We used this study as a way for predicting our human erythropoietin gene expression study in the CHO-K1 cells. In this study, we showed that expression of GFP decreased after incubation of the cells in selection medium containing G418. Expression of GFP seemed to be stable after about three weeks incubation in selection medium. Recombinant erythropoietin was also detected in the day 20. Key words: Ctionic lipid, CHO-K1 cells, erythropoietin, G418, green fluorescent protein

ISOLATION OF SULPHATE POLYGALACTAN FROM Eucheuma alvarezii Doty AND ITS ANTICOAGULANT ACTIVITY ., Sutrisno; ., Parlan; Al-Fath, Hanifah Nur
Indonesian Journal of Pharmacy Vol 24 No 2, 2013
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

A sulphate polygalactan carrageenan was isolated from Euchema alvarezii Doty with yield 54.36%. The sulphate polygalactan was fractionated with KCl (aq) 2.5% result of a sulphate polygalactan of soluble fraction with the yield 60.34% and a sulphate polygalactanof insoluble fraction with the yield 34.53%. Based on the properties and supported by IR spectrum, the sulphate polygalactan of soluble fraction is k-carrageenan, whilethe sulphate polygalactan of insoluble fraction is l-carrageenan. Anticoagulant activitiy of the carrageenans were based on their prolongation effects on Activated Partial Thromboplastine Time (APTT) and Prothrombine Time (PT) of sodium citrate. On the assays, the carrageenans exhibited promising anticoagulant activities.Key words: Eucheumaalvarezii Doty, k-carrageenan, l-carrageenan, anticoagulant activity, APTT and PT

EFFECT OF WATER MELON SEEDS EXTRACTS (Citrullus vulgaris) ON SPERMS IN DIABETIC RAT. Arash Khaki; Elaheh Ouladsahebmadarek; AfshinZahedi .; Behnam Ahmadnezhad
Indonesian Journal of Pharmacy Vol 24 No 2, 2013
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Citrullus vulgaris is an antioxidant and has been shown to reduce oxidative stress. Previous studies confirmed that antioxidants have essential effect on infertility through participating in reactive oxygen’s species. Chronic hyperglycemia is known to cause infertility in diabetes disease. Wistar male rats (n=40) were allocated into three groups: control group(n=10), Citrullus vulgaris seeds extract (CVE) group that received 55mg/kg by gavage method (n=10), and Diabetic group that received 55mg/kg (IP) streptozotocin (STZ) (n=20). The last group was subdivided into two groups of 10. STZ group and treatment group. Treatment group received 55mg/kg (IP) STZ plus 55mg/kg CVE, daily for 4weeks; however, the control group just received an equal volume of (0.9% NaCl) daily (gavage). Diabetes was induced by a single (IP) injection of streptozotocin (55mg/kg). Animals were kept in standard condition. In 28th day, 5cc bloodn sample was taken from every rat for biochemical analysis. Collecting epididymis tissues, they were prepared for sperm analysis by WHO method. In comparison to other groups, sperm parameters were significantly increased in groups that received 55mg/kg (CVE) (P

CONSTRUCTION OF IN SILICO STRUCTURE-BASED SCREENING TOOLS TO STUDY THE OXIDATIVE METABOLITES FORMATION OF CURCUMIN BY HUMAN CYTOCHROME 450 3A4 Dewi Setyaningsih; Muhammad Radifar; Yosi Bayu Murti; Enade Perdana Istyastono
Indonesian Journal of Pharmacy Vol 24 No 2, 2013
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Cytochrome P450 3A4 (CYP3A4) is a phase 1 metabolism enzyme which is responsible for the metabolism of about 3040% drug in the market. This CYP3A4 is the most abundant CYP450 expressed in human body and also the one who is responsible for the biotransformation of most drugs. The competitive inhibition of curcumin (a yellow bioactive pigment discovered in Curcuma sp.) towards human CYP3A4 indicates that curcumin can be a substrate for the enzyme. In this study, in silico approaches employing molecular docking and interaction fingerprinting were used to predict the binding mode and the site of metabolism (SOM) of curcumin. Together with the SOMs retrieved previously and the list of possible reactions catalyzed by CYP3A4, the docking and fingerprinting results indicate that the most probable metabolite of curcumin metabolism by human CYP3A4 is an oxidative metabolite 1-(3,4-dihydroxyphenyl)-5hydroxy-7-(4-hydroxy-3-methoxy-phenyl)hepta-1,4,6-trien-3one.Key words: site of metabolism (SOM), curcumin, biotransformation, in silico, molecular docking, protein-ligand interaction fingerprinting

ANTIANGIOGENESIS AND ANTIBACTERIAL ACTIVITIES FROM AN INDONESIAN MARINE-DERIVED FUNGUS Dactylaria sp Wilmar Maarisit; Dicki Huga Tangiono; Reinhard Pinontoan; Marstella Minelko; Joke Luis Tombuku; Tan Tjie Jan
Indonesian Journal of Pharmacy Vol 24 No 2, 2013
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Marine-derived fungi have been proven to be rich sources of chemically diverse natural products with a broad range of biological activities. The aim of this study was to determine the antibacterial and antiangiogenesis activities of marine derived fungi Dactylaria sp. Cultivation of the fungus Dactylaria sp (strain TID 24041021-1) was isolated from the marine invertebrate sponge. Culture of marine fungus was macerated with acetone and partitioned with ethyl acetate. The ethyl acetate extracts with 50, 100, and 200µg/mL concentrations, were assayed for their antiangiogenic activity by using chorioallantoic membrane in vivo. At the same time, ethyl acetate extracts at levels of 5, 10, 15, and 20mg/mL were assayed to pathogenic bacteria Bacillus subtilis, Escherichia coli, and Staphylococcus aureus using well diffusion method. The result of this study showed that ethyl acetate extract at concentration 50µg/mL could inhibit angiogenesis. The best antiangiogenic activity was showed at concentration of 200µg/mL ethyl acetate extract. Antibacterial activity from ethyl acetate extract inhibited the growth of B. subtilis (2.25-5mm), E. coli (0.63-3.50mm) and S. aureus (01.75mm) bacteria. Key words: Marine sponge-derived fungi, antiangiogenesis activity, antibacterial activity

CYTOTOXIC ACTIVITY OF SELECTED LIBYAN MEDICINAL PLANTS ON HUMAN BREAST ADENOCARCINOMA CELL LINE (MCF-7) Jamal, Elmezogi; Gazala, Ben Hussien; Mabrouk, Erhuma; Manal, Gadmour; Abdulmotaleb, Zetrini; Abossa, Abdolgader; Shilpi, Jamil Ahmad
Indonesian Journal of Pharmacy Vol 24 No 2, 2013
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Three Libyan plants namely Ballota pseudodictamnus, Hedera helix, Thapsia garganica were tested for anticancer activity. The plants, collected from Aljabal-Al-Akhdar area of Libya in April 2010 were shed dried and subjected to Soxhlet extraction by methanol. Phytochemical screening indicated the presence of 2-deoxy sugars, flavonoids, saponins and tannins but absence of alkaloids in all three plants under investigation. Antiproliferative activity was performed breast adenocarcinoma cell line (MCF7). At the concentration of 100µg/mL, B. pseudodictamnus, T. garganica and H. helix showed 90, 60 and 5% cell death, respectively as compared to the control. Key words: Antiproliferative; Breast adenocarcinoma; Cell l ine MCF-7

OXIDATION AND POLYMORPHISM OF FATTY ACIDS AND NUTRITIONAL ASPECTS CONJUGATED OF LINOLEIC ACIDS Mohammad Asif
Indonesian Journal of Pharmacy Vol 24 No 2, 2013
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Fats and oils are triglycerides containing one unit of glycerol with three units of fatty acids. It also contained mono- and diglycerides, phosphatides, cerebrosides, sterols, terpenes, fatty alcohols, free fatty acids, fat-soluble vitamins, and other substances. Fats and oils are recognized as essential nutrients in both human and animal diets. They provide the most concentrated source of energyand also provide essential fatty acids which are precursors for important hormones. Conjugated linoleic acid (CLA) is a group of geometrical and positional isomers of linoleic acid. In contrast to linoleic acid, double bonds in CLA are usually located at positions 9 and 11 or 10 and 12 and each double bond can be either in the cis or trans configuration. Meat and dairy products from ruminant animals are the principal natural sources of CLA in the human diet. Dietary CLA has been shown to have potent anti-carcinogenic, antiatherogenic, immune modulating and also have other biological activities. The CLA was also reported to reduce body fat content.Key words: anti-atherogenic, anticarcinogenic, conjugated linoleic acid, Fats and oils, immune modulator

IN VITRO ABSORPTION STUDY OF CARBAMAZEPINE SOLID DISPERSION USING EVERTED GUT SAC METHOD Thakur, Nishant; Thakral, Sunil; Goswami, Manish; Ghaie, Pankaj; Thakur, Amit; Mangal, Mohit
Indonesian Journal of Pharmacy Vol 24 No 2, 2013
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

The oral Bioavailability of BCS (Bio Pharmaceutical Classification System) class II drug with poor solubility and reasonable permeability is limited by drug dissolution. In order to improve the aqueous solubility of the drug and dissolution of thedrug, the solid dispersion was prepared and evaluated for its absorption in intestine using modified everted gut sac method. The solid dispersion of carbamezepine (CBZ) was prepared using polaxomer and guargum by kneading method. The CBZ and CBZSD (Solid Disposisi) shows 2.329% and 3.948% drug absorption, respectively. The data show that solid dispersion increase the absorption of the CBZ in CBZ-SD is more than 70% in comparison to pure CBZ. The increase in CBZ solubility of the SD could be attributed to several factors such as improved wettability, local solubilization, drug particle size reduction and crystalline or, interstitial solid solution reduction.Â Key words: Everted gut sac method, solid dispersion, absorption, solubility

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